Lentivirus Characterization

Direct Visualization of Lentivirus Formulations

Cryo-TEM or negative stain TEM imaging and analysis can provide valuable insights and assessment of CQA’s, and inform batch-to-batch variability, scale-up consistency, and process development changes in lentivirus formulations.

Quantitative & qualitative analysis (for R&D purposes or using validated methods) provide a wealth of answers using a single cryo-TEM dataset, only requiring ~50μl of sample.

Cryo-TEM Characterization of Lentiviral Vectors

Cryo-TEM imaging can be used for analysis of lentivirus characteristics, including:

Morphology (size, shape)
Particle uniformity
Particle integrity
Maturation state
Surface protein distribution
Presence of impurities

Cryo-electron tomography provides 3D volumes to better distinguish surface protein distribution and internal particle features.

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Other Viral & VLP Formulations we can Image with Cryo or NS TEM

microscopic black and white image of adeno-associated virus (AAV) characterization with cryo-TEM

Adeno-Associated Virus (AAV)

microscopic black and white image cryo-TEM imaging of adenovirus | nanoparticle characterization

Adenovirus

microscopic black and white image of Human Papillomavirus (HPV) Characterization by cryo-TEM | nanoimaging services NIS

Human Papillomavirus (HPV)

Vesicular Stomatitis Virus

Services Related to Lentivirus Characterization

Comprehensive Characterization of Lentivirus by Cryo or NS TEM

Lentivirus, a member of the retrovirus family, is a widely used viral vector in gene therapy.

One of its key advantages is its ability to transduce both dividing and non-dividing cells, making lentivirus vectors suitable for targeting diverse cell types including neurons, macrophages, hematopoietic stem cells, muscle and liver cells. Lentiviral vectors are generally low in immunogenicity and offer a favorable safety and efficacy profile compared to other viral vectors, supporting their broad use in therapeutic applications.

Another benefit is their relatively high packaging capacity, enabling delivery of larger genetic payloads than vectors like adeno-associated virus (AAV).

Lentiviral vectors are used in various fields including cancer research, vaccine development, and studies of drug resistance.

Cryo-TEM imaging and analysis supports every stage of lentiviral formulation development - from fast insights during R&D to validated, high-resolution data for regulatory filings.

Moreover, cryo-electron tomography (cryo-ET) enables 3D reconstructions of structurally diverse particle populations. These reconstructions help characterize individual particles within a formulation, offering detailed information on assembly, maturation, and potential activity of the lentivirus vector.

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